IVPT: in vitro patch tests

Laboratory methods in the detection of contact allergy - an overview of published studies

Important: If you are aware of a paper relevant to this topic that has not been included into this compilation, please let me know - you will find the contact email here. I will be more than happy to include previously unpublished negative results into this analysis - these are as important as "positive" results, yet we all know how difficult it is to get them published. In such case, please send me a description of your study for posting on this webpage in the form following rules for a standard manuscript with the aims, methods, results and conclusions (the introduction and discussion are welcome, yet not obligatory - a short comment summing up your experiments will suffice). The copyright for such manuscript shall remain with the authors.

Hapten

LPT

LBTT

ELISA

ELISPOT

FC

PCR

Nickel (Ni)

+[1], +[2], +[5], +[6], +[7], +[10], +[11], +[15], +[16], +[17], +[18], +[19], +[22], -[24], +[26], ?[27], -[28], +[29]

+[8]

IFN-γ: +[1], +[3], -[7], +[9], +[10], -[11], +[20], -[24], -[29]

IL-2: +[3], +[6], +[29]

IL-4: -[20], -[24], +[29]

IL-5: +[1], +[6], +[10], +[11], -[20]

IL-10: +[7], +[9], ?[10]

IL-13: +[3], +[9]

IL-17: -[7]

TGF-β: ?[10]

TNF-α: -[20]

TNF-β: +[29]

IFN-γ: +[8], -[11], -[23]

IL-2: +[3], +[8], +[11], -[23]

IL-4: +[3], +[8], +[9], -[23]

IL-5: +[9], +[11]

IL-10: -[23]

IL-12: -[23]

IL-13: +[3], +[9], +[11]

-[7]

(nd)

Chromium (Cr)

+[1], +[2], +[17], +[21], +[23], +[28]

(nd)

IFN-γ: +[1], ?[3], +[25]

IL-2: ?[3]

IL-5: +[1]

IL-13 + [3]

IL-2: +[3]

IL-4: ?[3]

IL-13: +[3]

(nd)

CASP8: +[2]

IFN-γ +[4]

IL-4: -[4]

IL-6: +[4]

Cobalt (Co)

+[1], +[5], +[17], -[28]

(nd)

IFN-γ: +[1], ?[3]

IL-2: ?[3]

IL-5: +[1]

IL-13: ?[3]

IL-2: ?[3]

IL-4: ?[3]

IL-13: ?[3]

(nd)

(nd)

Fragrances

+[1]

(nd)

IFN-γ: +[1]

IL-5: +[1]

(nd)

(nd)

(nd)

4-phenylenediamine (PPD)

+[1], +[12]

(nd)

IFN-γ: +[1], -[12]

IL-4: +[12]

IL-5: +[1], +[12]

(nd)

+[12]

?[12]

Methylchloroisothiazolinone (MCI)

+[1]

(nd)

IFN-γ: +[1]

IL-5: +[1]

(nd)

(nd)

(nd)

Parthenolide (Sesquiterpene lactone)

(nd)

(nd)

(nd)

IFN-γ: +[14]

IL-2: +[14]

IL-4: +[14]

IL-5: +[14]

IL-13: +[14]

(nd)

(nd)

Matricaria chamomilla L. aka Chamomilla recutita (ingredient of Compositae Mix)

+[13]

(nd)

(nd)

(nd)

(nd)

(nd)

Thiuram mix

+[15]

(nd)

(nd)

(nd)

(nd)

(nd)

Haptens beyond the European baseline series

Gold (Au)

+[19]

(nd)

IFN-γ: +[3]

IL-2: ?[3]

IL-13: +[3]

IL-2: ?[3]

IL-2: ?[3]

IL-13: ?[3]

(nd)

(nd)

Abbreviations: LPT - lymphocyte proliferation test (incorporation of 3H-thymidine into proliferating cells); LBTT - lymphocyte blastic transformation test (observation of blast formation under microscope); FC - flow cytometry; PCR - polymerase chain reaction; (nd) - no data.

References

  1. Moed H, von Blomberg M, Bruynzeel DP, Scheper R, Gibbs S, Rustemeyer T. Improved detection of allergen-specific T-cell responses in allergic contact dermatitis through the addition of 'cytokine cocktails'. Exp Dermatol 2005 Aug;14(8):634-40.
  2. Hansen MB, Skov L, Menné T, Olsen J. Gene transcripts as potential diagnostic markers for allergic contact dermatitis. Contact Dermatitis 2005 Aug;53(2):100-6.
  3. Minang JT, Areström I, Troye-Blomberg M, Lundeberg L, Ahlborg N. Nickel, cobalt, chromium, palladium and gold induce a mixed Th1- and Th2-type cytokine response in vitro in subjects with contact allergy to the respective metals. Clin Exp Immunol 2006 Dec;146(3):417-26.
  4. Thomas P, Summer B, Sander CA, Przybilla B, Thomas M, Naumann T. Intolerance of osteosynthesis material: evidence of dichromate contact allergy with concomitant oligoclonal T-cell infiltrate and TH1-type cytokine expression in the peri-implantar tissue. Allergy 2000 Oct;55(10):969-72.
  5. van den Broeke LT, Nilsson JL, Scheynius A, Wahlberg JE, Karlberg AT. Lack of antagonism to Ni2+ and Co2+ contact allergy from other essential divalent metal ions. Contact Dermatitis 1998 May;38(5):266-73.
  6. Buchvald D, Lundeberg L. Impaired responses of peripheral blood mononuclear cells to nickel in patients with nickel-allergic contact dermatitis and concomitant atopic dermatitis. Br J Dermatol 2004 Mar;150(3):484-92.
  7. Cederbrant K, Anderson C, Andersson T, Marcusson-Ståhl M, Hultman P. Cytokine production, lymphocyte proliferation and T-cell receptor Vbeta expression in primary peripheral blood mononuclear cell cultures from nickel-allergic individuals. Int Arch Allergy Immunol 2003 Dec;132(4):373-9.
  8. Lindemann M, Böhmer J, Zabel M, Grosse-Wilde H. ELISpot: a new tool for the detection of nickel sensitization. Clin Exp Allergy 2003 Jul;33(7):992-8.
  9. Minang JT, Troye-Blomberg M, Lundeberg L, Ahlborg N. Nickel elicits concomitant and correlated in vitro production of Th1-, Th2-type and regulatory cytokines in subjects with contact allergy to nickel. Scand J Immunol 2005 Sep;62(3):289-96.
  10. Rustemeyer T, von Blomberg BM, van Hoogstraten IM, Bruynzeel DP, Scheper RJ. Analysis of effector and regulatory immune reactivity to nickel. Clin Exp Allergy 2004 Sep;34(9):1458-66.
  11. Spiewak R, Moed H, von Blomberg BM, Bruynzeel DP, Scheper RJ, Gibbs S, Rustemeyer T. Allergic contact dermatitis to nickel: modified in vitro test protocols for better detection of allergen-specific response. Contact Dermatitis 2007 Feb;56(2):63-9.
  12. Sieben S, Kawakubo Y, Al Masaoudi T, Merk HF, Blömeke B. Delayed-type hypersensitivity reaction to paraphenylenediamine is mediated by 2 different pathways of antigen recognition by specific alphabeta human T-cell clones. J Allergy Clin Immunol 2002 Jun;109(6):1005-11.
  13. Stingeni L, Agea E, Lisi P, Spinozzi F. T-lymphocyte cytokine profiles in compositae airborne dermatitis. Br J Dermatol 1999 Oct;141(4):689-93.
  14. Wahlkvist H, Masjedi K, Gruvberger B, Zuber B, Karlberg AT, Bruze M, Ahlborg N. The lipophilic hapten parthenolide induces interferon-gamma and interleukin-13 production by peripheral blood-derived CD8+ T cells from contact allergic subjects in vitro. Br J Dermatol 2007 Jan;158(1):70-7.
  15. Kimber I, Quirke S, Cumberbatch M, Ashby J, Paton D, Aldridge R D, Hunter J A, Beck M H. Lymphocyte transformation and thiuram sensitization. Contact Dermatitis 1991 Mar;24(3):164-71.
  16. Svejgaard E, Morling N, Svejgaard A, Veien NK. Lymphocyte transformation induced by nickel sulphate: an in vitro study of subjects with and without a positive nickel patch test. Acta Derm Venereol. 1978;58(3):245-50.
  17. Al-Tawil NG, Marcusson JA, Möller E. HLA-class II restriction of the proliferative T lymphocyte responses to nickel, cobalt and chromium compounds. Tissue Antigens. 1985 Mar;25(3):163-72.
  18. Silvennoinen-Kassinen S. The specificity of a nickel sulphate reaction in vitro: a family study and a study of chromium-allergic subjects. Scand J Immunol. 1981;13(3):231-5.
  19. Cederbrant K, Hultman P, Marcusson JA, Tibbling L. In vitro lymphocyte proliferation as compared to patch test using gold, palladium and nickel. Int Arch Allergy Immunol. 1997 Mar;112(3):212-7.
  20. Borg L, Christensen JM, Kristiansen J, Nielsen NH, Menné Tand, Poulsen K. Nickel-induced cytokine production from mononuclear cells in nickel-sensitive individuals and controls. Cytokine profiles in nickel-sensitive individuals with nickel allergy-related hand eczema before and after nickel challenge. Arch Dermatol Res 2000 Jan; 292(6): 285-91.
  21. Räsänen L, Sainio H, Lehto M, Reunala T. Lymphocyte proliferation test as a diagnostic aid in chromium contact sensitivity. Contact Dermatitis. 1991 Jul;25(1):25-9.
  22. Räsänen L, Tuomi ML. Diagnostic value of the lymphocyte proliferation test in nickel contact allergy and provocation in occupational coin dermatitis. Contact Dermatitis. 1992 Oct;27(4):250-4.
  23. Lindemann M, Rietschel F, Zabel M, Grosse-Wilde H. Detection of chromium allergy by cellular in vitro methods. Clin Exp Allergy. 2008 Sep;38(9):1468-75.
  24. Lisby S, Hansen LH, Skov L, Menné T, Baadsgaard O. Nickel-induced activation of T cells in individuals with negative patch test to nickel sulphate. Arch Dermatol Res. 1999 May;291(5):247-52.
  25. Trattner A, Akerman L, Lapidoth M, Klein T, Weiss H, Ben Chaim B, David M. Use of in vitro release of interferon-gamma in the diagnosis of contact allergy to potassium dichromate - a controlled study. Contact Dermatitis. 2003 Apr;48(4):191-3.
  26. Hutchinson F, Raffle EJ, Macleod TM. The specificity of lymphocyte transformation in vitro by nickel salts in nickel sensitive subjects. J Invest Dermatol. 1972 Jun;58(6):362-5.
  27. Macleod TM, Hutchinson F, Raffle EJ. The uptake of labelled thymidine by leucocytes of nickel sensitive patients. Br J Dermatol. 1970 May;82(5):487-92.
  28. Gilboa R, Al-Tawil NG, Marcusson JA. Metal allergy in cashiers. An in vitro and in vivo study for the presence of metal allergy. Acta Derm Venereol. 1988;68(4):317-24.
  29. Falsafi-Amin H, Lundeberg L, Bakhiet M, Nordlind K. Early DNA synthesis and cytokine expression in the nickel activation of peripheral blood mononuclear cells in nickel-allergic subjects. Int Arch Allergy Immunol. 2000 Oct;123(2):170-6.

Copyright © 2009-2013 Radoslaw Spiewak
Document created: 4 June 2009, last updated: 21 September 2013.
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